@article{ref1,
title="Analysis of 4-Bromo-2,5-Dimethoxyphenethylamine abuser's urine: Identification and quantitation of urinary metabolites",
journal="Journal of forensic sciences",
year="2013",
author="Kanamori, Tatsuyuki and Nagasawa, Kyoko and Kuwayama, Kenji and Tsujikawa, Kenji and Iwata, Yuko T. and Inoue, Hiroyuki",
volume="58",
number="1",
pages="279-287",
abstract="The metabolites of 4-bromo-2,5-dimethoxyphenethylamine (2C-B), a psychoactive drug with hallucinogenic activity, were investigated in a urine sample from a user of 2C-B. The urine sample was deconjugated enzymatically and the metabolites were recovered by liquid-liquid extraction. The extract was analyzed by gas chromatography/mass spectrometry after derivatization, and the results were used to identify and quantitate the metabolites. 4-Bromo-2,5-dimethoxyphenylacetic acid was the most abundant metabolite of 2C-B in human urine and accounted for 73% of the total amount of detected metabolites, followed by 4-bromo-2-hydroxy-5-methoxyphenylacetic acid (13%) and 4-bromo-2,5-dimethoxyphenylethyl alcohol (4.5%). According to the literature, the main metabolites of 2C-B in rat urine are N-(4-bromo-2-methoxy-5-hydroxyphenylethyl)acetamide and N-(4-bromo-2-hydroxy-5-methoxyphenylethyl)acetamide. However, these metabolites accounted for only a small proportion of the total amount of detected metabolites in human urine, which indicates that there are significant species-specific differences in the metabolism of 2C-B. 4-Bromo-2,5-dimethoxyphenylacetic acid, which was the most abundant metabolite in human urine, is thought to be generated by deamination of 2C-B by monoamine oxidase (MAO) followed by oxidation by aldehyde dehydrogenase. Our results suggest that MAO plays a crucial role in the metabolism of 2C-B in humans.<p /> <p>Language: en</p>",
language="en",
issn="0022-1198",
doi="10.1111/j.1556-4029.2012.02289.x",
url="http://dx.doi.org/10.1111/j.1556-4029.2012.02289.x"
}