@article{ref1,
title="Altered expression pattern of testican-1 mRNA after brain injury",
journal="Biomedical research (Tokyo, Japan)",
year="2011",
author="Iseki, Ken and Hagino, Seita and Zhang, Yuxiang and Mori, Tetsuji and Sato, Nobuko and Yokoya, Sachihiko and Hozumi, Yasukazu and Goto, Kaoru and Tase, Choichiro",
volume="32",
number="6",
pages="373-378",
abstract="Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain. In situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. Testican-1 mRNA signals were detected in the proximal reactive astrocytes, whereas the distribution pattern of testican-1 mRNA positive cells was different from those of mature oligodendrocytes and activated microglia. In addition, signals for testican-1 mRNA overlapped with those of FGF-2 mRNA, showing that these molecules are coexpressed in reactive astrocytes. These results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury.<p /> <p>Language: en</p>",
language="en",
issn="0388-6107",
doi="",
url="http://dx.doi.org/"
}