@article{ref1,
title="Molecular diversity of the telson and venom components from Pandinus cavimanus (Scorpionidae Latreille 1802): Transcriptome, Venomics and Function",
journal="Proteomics",
year="2012",
author="Diego-Garcia, Elia and Peigneur, Steve and Clynen, Elke and Marien, Tessa and Czech, Lene and Schoofs, Liliane and Tytgat, Jan",
volume="12",
number="2",
pages="313-328",
abstract="Venom from the scorpion Pandinus cavimanus was obtained by electrical stimulation of the telson (stinger). Total venom was toxic to crickets at 7-30 μg and a paralysis or lethal effect was observed at 30 μg of venom (death at 1.5 μg / mg of cricket). Electrophysiological analyses showed cytolytic activity of total venom on oocytes at 7 μg. High pressure liquid chromatography (HPLC) allowed separation of the venom components. A total of thirty-eight fractions from total venom were tested on voltage-gated Na(+) and K(+) channels. Some fractions block K(+) currents in different degree. By using mass spectrometry (MS) analysis, we obtained more than 700 different molecular masses from telson and venom fractions (by LC-MS/MS and MALDI-TOF MS analyses). The number of disulfide bridges of the telson components was determined. A cDNA library from Pandinus cavimanus scorpion was constructed and a random sequencing screening of transcripts was conducted. Different clones were obtained and analyzed by bioinformatics tools. Our results reveal information of new genes related to some cellular processes and genes involved in venom gland functions (toxins, phospholipases and antimicrobial peptides). Expressed sequence tags from venom glands provide complementary information to mass spectrometry analysis and reveal undescribed components related to the biological activity of the venom.<p /> <p>Language: en</p>",
language="en",
issn="1615-9853",
doi="10.1002/pmic.201100409",
url="http://dx.doi.org/10.1002/pmic.201100409"
}