@article{ref1,
title="Determination of urinary 2,5-hexanedione concentration by an improved analytical method as an index of exposure to n-hexane",
journal="British journal of industrial medicine",
year="1991",
author="Saito, I. and Shibata, E. and Huang, Jing and Hisanaga, N. and Ono, Y. and Takeuchi, Y.",
volume="48",
number="8",
pages="568-574",
abstract="2,5-Hexanedione is a main metabolite of n-hexane and is considered as the cause of n-hexane polyneuropathy. Therefore, it is useful to measure 2,5-hexanedione for biological monitoring of exposure to n-hexane. The analytical methods existing for n-hexane metabolites, however, were controversial and not established enough. Hence, a simple and precise method for determination of urinary 2,5-hexanedione has been developed. Five ml of urine was acidified to pH 0.5 with concentrated hydrochloric acid and heated for 30 minutes at 90-100 degrees C. After cooling in water, sodium chloride and dichloromethane containing internal standard were added. The sample was shaken and centrifuged. 2,5-Hexanedione concentration in an aliquot of dichloromethane extract was quantified by gas chromatography using a widebore column (DB-1701). Urinary concentration of 2,5-hexanedione showed a good correlation with exposure to n-hexane (n = 50, r = 0.973, p less than 0.001). This method is simple and precise for analysis of urinary 2,5-hexanedione as an index of exposure to n-hexane.<p /><p>Language: en</p>",
language="en",
issn="0007-1072",
doi="",
url="http://dx.doi.org/"
}