@article{ref1,
title="A new method for the determination in blood and urine of a novel triazolobenzodiazepine (estazolam) by HPLC",
journal="Journal of analytical toxicology",
year="1986",
author="Scotto di Tella, A. and Ricci, P. and Di Nunzio, C. and Cassandro, P.",
volume="10",
number="2",
pages="65-67",
abstract="In this study the authors report a new method to determine estazolam in blood and urine by high pressure liquid chromatography (HPLC). This compound is a triazolobenzodiazepine and shows an interesting psychopharmacological activity. The extraction from biological fluids was carried out using a mixture of ethylenechloride, methylenechloride, and ethylacetate (1:1:8). The HPLC analysis was carried out with a C18 column, using methanol:phosphate buffer (pH 7.5, 0.011M):acetonitrile (65:33:2) as mobile phase. The detector was set at lambda = 240 nm. The method shows good repeatability and a linear response in the range of 0.6 to 10 micrograms estazolam/mL both in serum and urine. A case of non-lethal acute intoxication in which the method was applied is also reported.<p /> <p>Language: en</p>",
language="en",
issn="0146-4760",
doi="",
url="http://dx.doi.org/"
}